1. Single vesicle-vesicle fusion assay
M.Kyoung, A.Srivastava, Y.Zhang, J.Diao, M.Vrljic, P.Grob, E. Nogales, S.Chu, A.T.Brunger, In vitro system capable of differentiating fast calcium- triggered content mixing from lipid exchange for mechanistic studies of neurotransmitter release. Proc. Natl. Acad. Sci. USA 108, E304-E313 (2011)
J.Diao, P.Grob, D.J.Cipriano, M.Kyoung, Y.Zhang, S.Shah, A.Nguyen, M.Padolina, A.Srivastava, M.Vrljic, A.Shah, E.Nogales, S.Chu, A.T.Brunger. Synaptic proteins promote calcium-triggered fusion from point contract to full fusion. eLife 1, e00109 (2012).
M.Kyoung, Y.Zhang, J.Diao, S.Chu, A.T.Brunger. Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system. Nature Protocols 8, 1-16 (2013).

Primary data
2011_FUSION_SM01.mov (1.8 MB bytes): Movie S1, with neuronal SNAREs, synaptotagamin, and complexin.
2011_FUSION_SM02.mov (4 MB bytes): Movie S2, with neuronal SNAREs alone.
Snapshot of movie S1, with neuronal SNAREs, synaptotagmin, and complexin.